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Nerve reinnervation and itch behavior in a rat burn wound model
Author(s) -
Saffari Tiam M.,
Schüttenhelm Barthold N.,
van Neck Johan. W.,
Holstege Jan C.
Publication year - 2018
Publication title -
wound repair and regeneration
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.847
H-Index - 109
eISSN - 1524-475X
pISSN - 1067-1927
DOI - 10.1111/wrr.12620
Subject(s) - reinnervation , calcitonin gene related peptide , scratching , medicine , anesthesia , tyrosine hydroxylase , wound healing , calcitonin , endocrinology , anatomy , immunohistochemistry , neuropeptide , surgery , physics , receptor , acoustics
In this study, we investigated whether postburn itch in rats, after a full thickness burn, is correlated to the nervous reinnervation of the burn wound area. For this purpose, we determined scratching duration (expressed as second/hour) at 24 hours, 2, 4, 8, and 12 weeks postburn and combined this with immunohistochemistry for protein gene product 9.5 (PGP9.5) to identify all nerve fibers, calcitonin gene related peptide (CGRP) to identify peptidergic fibers, tyrosine hydroxylase (TH) for sympathetic fibers, and growth‐associated protein 43 (GAP‐43) for regrowing fibers. We found a modest, but highly significant, increase in scratching duration of all burn wound rats from 3 to 12 weeks postburn (maximally 63 ± 9.5 second/hour compared to sham 3.1 ± 1.4 second/hour at 9 weeks). At 24 hours postburn, all nerve fibers had disappeared from the burn area. Around 4 weeks postburn PGP 9.5‐ and CGRP‐immunoreactive nerve fibers returned to control levels. TH‐ and GAP‐43‐IR nerve fibers, which we found to be almost completely colocalized, did not regrow. No correlation was found between scratching duration and nervous reinnervation of the skin. The present results suggest that in rat, like in human, burn wound healing will induce increased scratching, which is not correlated to the appearance of nervous reinnervation.