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Inhibition of TGF‐β signaling promotes expansion of human epidermal keratinocytes in feeder cell co‐culture
Author(s) -
Suzuki Daisuke,
Pinto Filipa,
Senoo Makoto
Publication year - 2017
Publication title -
wound repair and regeneration
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.847
H-Index - 109
eISSN - 1524-475X
pISSN - 1067-1927
DOI - 10.1111/wrr.12541
Subject(s) - transforming growth factor , in vitro , keratinocyte , human skin , microbiology and biotechnology , limiting , epidermal growth factor , wound healing , cancer research , epidermis (zoology) , cell culture , signal transduction , biology , immunology , anatomy , biochemistry , genetics , mechanical engineering , engineering
Cultured epidermal autografts have been used worldwide since 1981 for patients with extensive third‐degree burn wounds and limited skin donor sites. Despite significant progress in techniques toward improving clinical outcome of skin grafts, the long in vitro preparation time of cultured autografts has remained a major factor limiting its widespread use. Here, we show that pharmacological inhibition of TGF‐β signaling promotes the expansion of human epidermal keratinocytes (HEKs) with high proliferative potential in co‐cultures with both murine 3T3‐J2 cells and human feeder cells, including dermal fibroblasts and preadipocytes. In contrast, TGF‐β signaling inhibition does not enhance the growth of HEKs in a serum‐ and feeder‐free condition, an alternative approach to propagate HEKs for subsequent autograft production. Our results have important implications for the use of TGF‐β signaling inhibition as a viable therapeutic strategy for improving Green's methodology and for more efficient production of customized skin autografts with human feeder cells.