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GHK‐Cu‐liposomes accelerate scald wound healing in mice by promoting cell proliferation and angiogenesis
Author(s) -
Wang Xinying,
Liu Baoquan,
Xu Qiang,
Sun Haiyang,
Shi Meijun,
Wang Dan,
Guo Meihua,
Yu Jiawen,
Zhao Chunhui,
Feng Bin
Publication year - 2017
Publication title -
wound repair and regeneration
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.847
H-Index - 109
eISSN - 1524-475X
pISSN - 1067-1927
DOI - 10.1111/wrr.12520
Subject(s) - liposome , wound healing , angiogenesis , cd31 , chemistry , flow cytometry , umbilical vein , cell growth , fibroblast , cell cycle , pharmacology , cell , microbiology and biotechnology , medicine , immunology , cancer research , biology , biochemistry , in vitro
Glycyl‐ l ‐histidyl‐ l ‐lysine (GHK)‐Cu is considered to be an activator of tissue remodeling, and has been used in cosmetic products. In this study, we prepared liposomes encapsulating GHK‐Cu and analyzed their effect on human umbilical vein endothelial cells (HUVECs) proliferation and scald wound healing in mice. The nanoscaled GHK‐Cu‐liposomes promoted HUVECs proliferation, with a 33.1% increased rate. Flow cytometry analysis showed increased cell number at G1 stage and decreased cell number at G2 stage after GHK‐Cu‐liposomes treatment. Western blotting indicated that the expression of vascular endothelial growth factor and fibroblast grow factors‐2 were both enhanced, as well as cell cycle‐related proteins CDK4 and CyclinD1. In a mice scald model, angiogenesis in burned skin treated with GHK‐Cu‐liposomes was better compared with free GHK‐Cu, and immunofluorescence analysis showed enhanced signal of CD31 and Ki67 in GHK‐Cu‐liposomes treated mice. Moreover, the wound healing time was shortened to 14 days post injury. Our results provide the evidence that GHK‐Cu‐liposomes could be utilized as a treatment for skin wounds.