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Adipose‐derived aldehyde dehydrogenase–expressing cells promote dermal regenerative potential with collagen‐glycosaminoglycan scaffold
Author(s) -
Matsumine Hajime,
Numakura Kazuyuki,
Tsunoda Satoshi,
Wang Huan,
Matsumine Rui,
Climov Mihail,
Giatsidis Giorgio,
Sukhatme Vikas P.,
Orgill Dennis P.
Publication year - 2017
Publication title -
wound repair and regeneration
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.847
H-Index - 109
eISSN - 1524-475X
pISSN - 1067-1927
DOI - 10.1111/wrr.12494
Subject(s) - adipose tissue , aldehyde dehydrogenase , chemistry , stromal vascular fraction , wound healing , stem cell , transplantation , stromal cell , glycosaminoglycan , pathology , microbiology and biotechnology , medicine , biology , biochemistry , immunology , surgery , enzyme
Aldehyde dehydrogenase (ALDH) is an enzyme that plays an important role in retinoid metabolism and highly expressed in stem cells. This study isolated ALDH‐expressing cells from subcutaneous adipose tissue and investigated their potential to enhance healing in a full‐thickness skin wound in rats by co‐implanting them with collagen‐glycosaminoglycan (c‐GAG) scaffolds. ALDH‐positive cells were isolated by a fluorescence‐activated cell sorting technique from Lewis rat's stromal‐vascular‐fraction (SVF) and transplanted with c‐GAG scaffolds in a rat full‐thickness skin wound model. At 7 days after surgery, the microscopic appearance of c‐GAG scaffolds seeded with ALDH‐positive was compared with those of uncultured‐SVF, and cultured‐SVF adipose‐derived stromal cells (ASCs). The thickness of cellular ingrowth in the ASC group (630 ± 180 μm) was significantly thicker than that in the control (390 ± 120 μm) or SVF (380 ± 140 μm) groups, but non‐significantly thicker than that in the ALDH‐positive group (570 ± 220 μm). The thickness of regenerated collagen layer was significantly thicker in the ALDH‐positive group (160 ± 110 μm) than in the ASCs (81 ± 41 μm), the control (65 ± 24 μm), or SVF (64 ± 34 μm) groups. Immunofluorescent staining with CD31 proved that transplanted ALDH‐positive cells differentiated into vascular endothelial cells in c‐GAG scaffolds. Combined transplantation with c‐GAG scaffolds and adipose‐derived ALDH‐positive cells promoted dermal regeneration, giving a possibility that ALDH‐positive cells would greatly shorten the waiting period before secondary autologous skin grafting was possible.