Premium
Inhibition of Pseudomonas aeruginosa biofilm formation on wound dressings
Author(s) -
Brandenburg Kenneth S.,
Calderon Diego F.,
Kierski Patricia R.,
Brown Amanda L.,
Shah Nihar M.,
Abbott Nicholas L.,
Schurr Michael J.,
Murphy Christopher J.,
McAnulty Jonathan F.,
Czuprynski Charles J.
Publication year - 2015
Publication title -
wound repair and regeneration
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.847
H-Index - 109
eISSN - 1524-475X
pISSN - 1067-1927
DOI - 10.1111/wrr.12365
Subject(s) - biofilm , pseudomonas aeruginosa , microbiology and biotechnology , wound healing , chronic wound , chemistry , crystal violet , tryptophan , bacteria , medicine , biology , amino acid , surgery , biochemistry , genetics
Chronic nonhealing skin wounds often contain bacterial biofilms that prevent normal wound healing and closure and present challenges to the use of conventional wound dressings. We investigated inhibition of Pseudomonas aeruginosa biofilm formation, a common pathogen of chronic skin wounds, on a commercially available biological wound dressing. Building on prior reports, we examined whether the amino acid tryptophan would inhibit P. aeruginosa biofilm formation on the three‐dimensional surface of the biological dressing. Bacterial biomass and biofilm polysaccharides were quantified using crystal violet staining or an enzyme linked lectin, respectively. Bacterial cells and biofilm matrix adherent to the wound dressing were visualized through scanning electron microscopy. d ‐/ l ‐tryptophan inhibited P. aeruginosa biofilm formation on the wound dressing in a dose dependent manner and was not directly cytotoxic to immortalized human keratinocytes although there was some reduction in cellular metabolism or enzymatic activity. More importantly, d ‐/ l ‐tryptophan did not impair wound healing in a splinted skin wound murine model. Furthermore, wound closure was improved when d ‐/ l ‐tryptophan treated wound dressing with P. aeruginosa biofilms were compared with untreated dressings. These findings indicate that tryptophan may prove useful for integration into wound dressings to inhibit biofilm formation and promote wound healing.