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Self‐synthesized extracellular matrix contributes to mature adipose tissue regeneration in a tissue engineering chamber
Author(s) -
Zhan Weiqing,
Chang Qiang,
Xiao Xiaolian,
Dong Ziqing,
Zeng Zhaowei,
Gao Jianhua,
Lu Feng
Publication year - 2015
Publication title -
wound repair and regeneration
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.847
H-Index - 109
eISSN - 1524-475X
pISSN - 1067-1927
DOI - 10.1111/wrr.12292
Subject(s) - adipose tissue , adipogenesis , extracellular matrix , stromal cell , tissue engineering , microbiology and biotechnology , regeneration (biology) , stromal vascular fraction , adipose tissue macrophages , connective tissue , chemistry , biology , biomedical engineering , pathology , white adipose tissue , medicine , cancer research , biochemistry
ABSTRACT The development of an engineered adipose tissue substitute capable of supporting reliable, predictable, and complete fat tissue regeneration would be of value in plastic and reconstructive surgery. For adipogenesis, a tissue engineering chamber provides an optimized microenvironment that is both efficacious and reproducible; however, for reasons that remain unclear, tissues regenerated in a tissue engineering chamber consist mostly of connective rather than adipose tissue. Here, we describe a chamber‐based system for improving the yield of mature adipose tissue and discuss the potential mechanism of adipogenesis in tissue‐chamber models. Adipose tissue flaps with independent vascular pedicles placed in chambers were implanted into rabbits. Adipose volume increased significantly during the observation period (week 1, 2, 3, 4, 16). Histomorphometry revealed mature adipose tissue with signs of adipose tissue remolding. The induced engineered constructs showed high‐level expression of adipogenic (peroxisome proliferator‐activated receptor γ), chemotactic (stromal cell‐derived factor 1a), and inflammatory (interleukin 1 and 6) genes. In our system, the extracellular matrix may have served as a scaffold for cell migration and proliferation, allowing mature adipose tissue to be obtained in a chamber microenvironment without the need for an exogenous scaffold. Our results provide new insights into key elements involved in the early development of adipose tissue regeneration.