Premium
Utilization of laser D oppler flowmetry and tissue spectrophotometry for burn depth assessment using a miniature swine model
Author(s) -
Lotter Oliver,
Held Manuel,
Schiefer Jennifer,
Werner Ole,
Medved Fabian,
Schaller HansEberhard,
RahmanianSchwarz Afshin,
Jaminet Patrick,
Rothenberger Jens
Publication year - 2015
Publication title -
wound repair and regeneration
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.847
H-Index - 109
eISSN - 1524-475X
pISSN - 1067-1927
DOI - 10.1111/wrr.12246
Subject(s) - laser doppler velocimetry , medicine , hemoglobin , oxygenation , h&e stain , burn injury , blood flow , biomedical engineering , laser light , nuclear medicine , staining , laser , surgery , pathology , anesthesia , optics , physics
Abstract Currently, the diagnosis of burn depth is primarily based on a visual assessment and can be dependent on the surgeons' experience. The goal of this study was to determine the ability of laser D oppler flowmeter combined with a tissue spectrophotometer to discriminate burn depth in a miniature swine burn model. Burn injuries of varying depth, including superficial‐partial, deep‐partial, and full thickness, were created in seven G öttingen minipigs using an aluminium bar (100 ° C ), which was applied to the abdominal skin for periods of 1, 3, 6, 12, 30, and 60 seconds with gravity alone. The depth of injury was evaluated histologically using hematoxylin and eosin staining. All burns were assessed 3 hours after injury using a device that combines a laser light and a white light to determine blood flow, hemoglobin oxygenation, and relative amount of hemoglobin. The blood flow (41 vs. 124 arbitrary units [ AU ]) and relative amount of hemoglobin (32 vs. 52 AU ) were significantly lower in full thickness compared with superficial‐partial thickness burns. However, no significant differences in hemoglobin oxygenation were observed between these depths of burns (61 vs. 60%). These results show the ability of laser D oppler flowmeter and tissue spectrophotometer in combination to discriminate between various depths of injury in the minipig model, suggesting that this device may offer a valuable tool for burn depth assessment influencing burn management.