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Self‐assembled adult adipose‐derived stem cell spheroids combined with biomaterials promote wound healing in a rat skin repair model
Author(s) -
Hsu Shanhui,
Hsieh PaiShan
Publication year - 2015
Publication title -
wound repair and regeneration
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.847
H-Index - 109
eISSN - 1524-475X
pISSN - 1067-1927
DOI - 10.1111/wrr.12239
Subject(s) - angiogenesis , microbiology and biotechnology , spheroid , wound healing , mesenchymal stem cell , adipose tissue , stem cell , paracrine signalling , chemistry , neovascularization , fibroblast , immunology , biology , cancer research , in vitro , receptor , biochemistry
Adult adipose‐derived stem cells ( ASC s) are a type of multipotent mesenchymal stem cells ( MSC s) with easy availability and serve as a potential cell source for cell‐based therapy. Three‐dimensional MSC spheroids may be derived from the self‐assembly of individual MSC s grown on certain polymer membranes. In this study, we demonstrated that the self‐assembled ASC spheroids on chitosan‐hyaluronan membranes expressed more cytokine genes (fibroblast growth factor 1, vascular endothelial growth factor, and chemokine [C‐C motif] ligand 2) as well as migration‐associated genes (chemokine [C‐X‐C motif] receptor type 4 and matrix metalloprotease 1) compared with ASC dispersed single cells grown on culture dish. To evaluate the in vivo effects of these spheroids, we applied ASC single cells and ASC spheroids in a designed rat skin repair model. Wounds of 15 × 15 mm were created on rat dorsal skin, where ASCs were administered and covered with hyaluronan gel/chitosan sponge to maintain a moist environment. Results showed that skin wounds treated with ASC spheroids had faster wound closure and a significantly higher ratio of angiogenesis. Tracking of fluorescently labeled ASC s showed close localization of ASC spheroids to microvessels, suggesting enhanced angiogenesis through paracrine effects. Based on the in vitro and in vivo results, the self‐assembled ASC spheroids may be a promising cellular source for skin tissue engineering and wound regeneration.