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The role of micro RNA ‐15 b in the impaired angiogenesis in diabetic wounds
Author(s) -
Xu Junwang,
Zgheib Carlos,
Hu Junyi,
Wu Wenjie,
Zhang Liping,
Liechty Kenneth W.
Publication year - 2014
Publication title -
wound repair and regeneration
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.847
H-Index - 109
eISSN - 1524-475X
pISSN - 1067-1927
DOI - 10.1111/wrr.12217
Subject(s) - angiogenesis , wound healing , medicine , microrna , mesenchymal stem cell , pathogenesis , gene expression , cancer research , immunology , gene , pathology , biology , genetics
The impairment in diabetic wound healing represents a significant clinical problem. Decreased angiogenesis is thought to play a central role in the pathogenesis of this impairment. We have previously shown that treatment of diabetic murine wounds with mesenchymal stem cells can improve healing, but the mechanisms are not completely defined. Micro RNA ‐15 b (mi R ‐15 b ) has been implicated in the regulation of the angiogenic response. We hypothesized that abnormal mi R ‐15 b expression may contribute to the impaired angiogenesis observed in impaired diabetic wound healing. To test this hypothesis, we examined the expression of mi R ‐15 b and its target genes in diabetic and nondiabetic mice before and after injury. Mi R ‐15 b expression was significantly up‐regulated in diabetic mouse wounds during the wound healing response. Increased mi R ‐15 b levels also closely correlated with decreased gene expression of its proangiogenic target genes. Furthermore, the correction of the diabetic wound healing impairment with mesenchymal stem cell treatment was associated with a significant decrease in mi R ‐15 b expression level and increased gene expression of its proangiogenic target genes. These results provide the first evidence that increased expression of mi R ‐15 b in diabetic wounds in response to injury may, in part, be responsible for the abnormal angiogenic response seen in diabetic wounds and may contribute to the observed wound healing impairment.