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Inhibition of poly‐ ADP ribose polymerase enzyme activity prevents hyperglycemia‐induced impairment of angiogenesis during wound healing
Author(s) -
Sarras Michael P.,
Mason Samantha,
McAllister Geoffrey,
Intine Robert V.
Publication year - 2014
Publication title -
wound repair and regeneration
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.847
H-Index - 109
eISSN - 1524-475X
pISSN - 1067-1927
DOI - 10.1111/wrr.12216
Subject(s) - angiogenesis , zebrafish , wound healing , diabetes mellitus , medicine , endocrinology , enzyme , biology , biochemistry , immunology , gene
We previously reported a zebrafish model of type I diabetes mellitus ( DM ) that can be used to study the hyperglycemic ( HG ) and metabolic memory ( MM ) states within the same fish. Clinically, MM is defined as the persistence of diabetic complications even after glycemic control is pharmacologically achieved. In our zebrafish model, MM occurs following β‐cell regeneration, which returns fish to euglycemia. During HG , fish acquire tissue deficits reflective of the complications seen in patients with DM and these deficits persist after fish return to euglycemia ( MM ). The unifying mechanism for the induction of diabetic complications involves a cascade of events that is initiated by the HG stimulation of poly‐ ADP ribose polymerase enzyme ( P arp) activity. Additionally, recent evidence shows that the HG induction of P arp activity stimulates changes in epigenetic mechanisms that correlate with the MM state and the persistence of complications. Here we report that wound‐induced angiogenesis is impaired in DM and remains impaired when fish return to a euglycemic state. Additionally, inhibition of P arp activity prevented the HG ‐induced wound angiogenesis deficiency observed. This approach can identify molecular targets that will provide potential new avenues for therapeutic discovery as angiogenesis imbalances are associated with all HG ‐damaged tissues.