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Hyaluronan enhances wound repair and increases collagen III in aged dermal wounds
Author(s) -
Damodarasamy Mamatha,
Johnson Richard S.,
Bentov Itay,
MacCoss Michael J.,
Ver Robert B.,
Reed May J.
Publication year - 2014
Publication title -
wound repair and regeneration
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.847
H-Index - 109
eISSN - 1524-475X
pISSN - 1067-1927
DOI - 10.1111/wrr.12192
Subject(s) - glycosaminoglycan , extracellular matrix , wound healing , hyaluronic acid , blot , chemistry , extracellular , matrix (chemical analysis) , microbiology and biotechnology , biochemistry , surgery , medicine , anatomy , biology , gene , chromatography
Age‐related changes in the extracellular matrix contribute to delayed wound repair in aging. Hyaluronan, a linear nonsulfated glycosaminoglycan, promotes synthesis and assembly of key extracellular matrix components, such as the interstitial collagens, during wound healing. The biological effects of hyaluronan are mediated, in part, by hyaluronan size. We have previously determined that dermal wounds in aged mice, relative to young mice, have deficits in the generation of lower molecular weight hyaluronan (defined as <300 kDa ). Here, we tested the effect of exogenous hyaluronan of 2, 250, or 1,000 kDa sizes on full‐thickness excisional wounds in aged mice. Only wounds treated with 250 kDa hyaluronan ( HA 250) were significantly improved over wounds that received carrier (water) alone. Treatment with HA 250 was associated with increased expression of transcripts for the hyaluronan receptors CD 44 and RHAMM , as well as collagens III and I . Analyses of dermal protein content by mass spectrometry and W estern blotting confirmed significantly increased expression of collagen III in wounds treated with HA 250 relative to control wounds. In summary, we find that HA 250 improves wound repair and increases the synthesis of collagen III in aged dermal wounds.