Premium
A novel decontaminant and wound healant formulation of N , N ′‐dichloro‐bis[2,4,6‐trichlorophenyl]urea against sulfur mustard–induced skin injury
Author(s) -
Lomash Vinay,
Pant Satish C.
Publication year - 2014
Publication title -
wound repair and regeneration
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.847
H-Index - 109
eISSN - 1524-475X
pISSN - 1067-1927
DOI - 10.1111/wrr.12126
Subject(s) - wound healing , chemistry , angiogenesis , sulfur mustard , hydroxyproline , vascular endothelial growth factor , pharmacology , food science , biochemistry , surgery , medicine , toxicity , vegf receptors , organic chemistry , cancer research
Sulfur mustard ( SM )–induced dermatotoxicity can be prevented by an immediate use of decontamination agents. However, practically due to the time lapse between decontamination and exposure, there is always a possibility of wound formation. In view of this, a hydrophilic decontamination formulation of CC ‐2 ( DRDE / WH ‐03) was fortified with A loe vera gel and betaine ( DRDE / WH ‐01) for improving its wound healing ability. Swiss albino mice were exposed to SM percutaneously (5 mg/kg) once, and after 24 hours, DRDE / WH ‐01, DRDE / WH ‐03, framycetin, and aloe gel were applied topically, daily for 7 days. Skin sections were subjected to histopathology, histomorphologic grading, tissue leukocytosis, and immunohistochemistry of inflammatory‐reparative biomarkers on 3 and 7 days, respectively. DRDE / WH ‐01, framycetin, and aloe gel showed better reepithelialization, angiogenesis, and fibroplasia compared with DRDE / WH ‐03 and SM control. On the basis of histomorphologic scale, DRDE / WH ‐01, framycetin, and aloe gel were found to be equally efficacious. Up‐regulation of interleukin‐6 and infiltrating leukocytes, endothelial nitric oxide synthase and angiogenesis, fibroblast growth factor, and transforming growth factor‐alpha with fibroplasia and reepithelialization were well correlated at various stages of the healing process. DRDE / WH ‐01 was equally effective as framycetin and has shown improved wound healing efficacy compared with DRDE / WH ‐03. Thus, DRDE / WH ‐01 can be recommended as a universal decontaminant and wound healant against vesicant‐induced skin injury.