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Effects of systemic pretreatment with CpG oligodeoxynucleotides on skin wound healing in mice
Author(s) -
Hergert Bettina,
Grambow Eberhard,
Butschkau Antje,
Vollmar Brigitte
Publication year - 2013
Publication title -
wound repair and regeneration
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.847
H-Index - 109
eISSN - 1524-475X
pISSN - 1067-1927
DOI - 10.1111/wrr.12084
Subject(s) - cpg oligodeoxynucleotide , wound healing , keratinocyte , inflammation , fibroblast , immune system , cpg site , chemistry , receptor , microbiology and biotechnology , immunology , biology , in vitro , gene expression , biochemistry , dna methylation , gene
Unmethylated CpG oligodeoxynucleotides ( ODN ) bind to the T oll‐like receptor 9, thus stimulating the immune system. To study the effects of systemic pretreatment with CpG ODN on dermal regeneration, C57BL6/J T yr mice were treated with CpG or control ODN 6 days prior to implantation of a dorsal skinfold chamber and skin wounding. Wound epithelialization was analyzed by planimetric microscopy. On day 18, wound tissues were taken for (immuno)histochemical staining. CpG ODN increased epithelialization compared with control ODN treatment. Histological analysis revealed reduced capillary density, reduced wound cellularity, and reduced numbers of infiltrating leukocytes, as well as reduced F4 /80‐positive macrophages, but increased numbers of RELM ‐α‐positive M2 macrophages after CpG ODN treatment, reflecting a better quality of wound healing on day 18 compared with control ODN treatment. Reverse transcription‐polymerase chain reaction analysis of T oll‐like receptor 9 showed the receptor expression on both fibroblasts and keratinocytes. Fibroblasts showed an increase of migration upon increasing dosages of CpG and not control ODN , reaching ∼50% of the response of basic fibroblast growth factor‐exposed cells. Keratinocytes dose‐dependently responded to both CpG and control ODN up to values found in keratinocyte growth factor‐exposed cells. In summary, CpG ODN support late tissue‐remodeling processes that contribute to resolution of inflammation and solid wounds during skin regeneration.