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Topical application of the lectin A rtin M accelerates wound healing in rat oral mucosa by enhancing TGF ‐β and VEGF production
Author(s) -
Kim Yeon J.,
Carvalho Fernanda C.,
Souza João A.C.,
Gonçalves Pedro C.G.,
Nogueira Andressa V.B.,
Spolidório Luis C.,
RoqueBarreira Maria C.,
Cirelli Joni A.
Publication year - 2013
Publication title -
wound repair and regeneration
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.847
H-Index - 109
eISSN - 1524-475X
pISSN - 1067-1927
DOI - 10.1111/wrr.12041
Subject(s) - wound healing , vascular endothelial growth factor , chemistry , oral mucosa , immunohistochemistry , transforming growth factor , andrology , pathology , vegf receptors , immunology , medicine , endocrinology
The lectin A rtin M has been shown to accelerate the wound‐healing process. The aims of this study were to evaluate the effects of A rtin M on wound healing in the palatal mucosa of rats and to investigate the effects of A rtin M on transforming growth factor beta ( TGF ‐β) and vascular endothelial growth factor ( VEGF ) secretion by rat gingival fibroblasts. A surgical wound was created on the palatal mucosa of 72 rats divided into three groups according to treatment: C — C ontrol (nontreated), A — A rtin M gel, and V — V ehicle. Eight animals per group were sacrificed at 3, 5, and 7 days postsurgery for histology, immunohistochemistry and determination of the levels of cytokines, and growth factors. Gingival fibroblasts were incubated with 2.5 μg/m L of A rtin M for 24, 48, and 72 hours. The expression of VEGF and TGF ‐β was determined by enzyme‐linked immunosorbent assay. Histologically, at day 7, the A rtin M group showed earlier reepithelialization, milder inflammatory infiltration, and increased collagen fiber formation, resulting in faster maturation of granular tissue than in the other groups ( p < 0.05). A rtin M–induced cell proliferation in vivo and promoted a greater expression of TGF ‐β and VEGF in both experiments ( p < 0.05). A rtin M was effective in healing oral mucosa wounds in rats and was associated with increased TGF ‐β and VEGF release, cell proliferation, reepithelialization, and collagen deposition and arrangement of fibers.