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A modified collagen gel enhances healing outcome in a preclinical swine model of excisional wounds
Author(s) -
Elgharably Haytham,
Roy Sashwati,
Khanna Savita,
Abas Motaz,
DasGhatak Piya,
Das Amitava,
Mohammed Kareem,
Sen Chandan K.
Publication year - 2013
Publication title -
wound repair and regeneration
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.847
H-Index - 109
eISSN - 1524-475X
pISSN - 1067-1927
DOI - 10.1111/wrr.12039
Subject(s) - wound healing , medicine , inflammation , masson's trichrome stain , pathology , staining , surgery , immunology
Collagen‐based dressings are of great interest in wound care. However, evidence supporting their mechanism of action is scanty. This work provides first results from a preclinical swine model of excisional wounds, elucidating the mechanism of action of a modified collagen gel (MCG) dressing. Following wounding, wound‐edge tissue was collected at specific time intervals (3, 7, 14, and 21 days postwounding). On day 7, histological analysis showed significant increase in the length of rete ridges, suggesting improved biomechanical properties of the healing wound tissue. Rapid and transient mounting of inflammation is necessary for efficient healing. MCG significantly accelerated neutrophil and macrophage recruitment to the wound site on day 3 and day 7 with successful resolution of inflammation on day 21. MCG induced monocyte chemotactic protein‐1 expression in neutrophil‐like human promyelocytic leukemia‐60 cells in vitro. In vivo, MCG‐treated wound tissue displayed elevated vascular endothelial growth factor expression. Consistently, MCG‐treated wounds displayed significantly higher abundance of endothelial cells with increased blood flow to the wound area indicating improved vascularization. This observation was explained by the finding that MCG enhanced proliferation of wound‐site endothelial cells. In MCG‐treated wound tissue, Masson's trichrome and picrosirius red staining showed higher abundance of collagen and increased collagen type I:III ratio. This work presents first evidence from a preclinical setting explaining how a collagen‐based dressing may improve wound closure by targeting multiple key mechanisms. The current findings warrant additional studies to determine whether the responses to the MCG are different from other collagen‐based products used in clinical setting.