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Optimization of immobilization process and survival study of microbial sensing strains used for aromatic hydrocarbon detection in industrial wastewater
Author(s) -
Patel Rushika,
Mukherjee Anwesha,
Zaveri Purvi,
Mehta Krupa,
Aswani Himani,
Munshi Nasreen S.
Publication year - 2020
Publication title -
water and environment journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.437
H-Index - 37
eISSN - 1747-6593
pISSN - 1747-6585
DOI - 10.1111/wej.12604
Subject(s) - wastewater , biosensor , biodegradation , pollutant , aromatic hydrocarbon , industrial wastewater treatment , chemistry , hydrocarbon , agarose , pseudomonas , calcium alginate , environmental chemistry , strain (injury) , chromatography , bacteria , environmental engineering , environmental science , biology , organic chemistry , biochemistry , calcium , anatomy , genetics
A variety of biosensing strains for monitoring of aromatic hydrocarbon pollutants have been developed but only a few have been applied to the field due to their lower survival rate and susceptibility to toxic pollutants. Immobilization is one of the solutions to protect strain from toxic pollutants. Synthetic and natural matrices were screened for the immobilization of three strains, which were able to detect one‐, two‐ and three‐ring aromatic hydrocarbon compounds. Agarose and calcium alginate were found to be the best matrices for strain immobilization. The survival rate of immobilized strains was determined by the colony‐forming unit (CFU) count at regular intervals till it reaches to the non‐detectable level. The stability of immobilized biosensing strains was found to be 12–15 days in the industrial wastewater. Hence vector from strains was transformed into wastewater isolate Pseudomonas citronellolis . Immobilized Pseudomonas citronellolis was stable for 66 days in industrial wastewater. Immobilized strains may be applied to design automated biosensor for the online monitoring of pollutants.