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Temporal responses of 4‐chlorophenol on the performance and functional genes expression of activated sludge in sequencing batch bioreactor
Author(s) -
Zhao Jianguo,
Li Yahe,
Li Yu,
Yang Dejian,
Zhu Changai,
Zhang Shanqing
Publication year - 2020
Publication title -
water and environment journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.437
H-Index - 37
eISSN - 1747-6593
pISSN - 1747-6585
DOI - 10.1111/wej.12585
Subject(s) - sequencing batch reactor , pollutant , activated sludge , hydraulic retention time , bioreactor , chemistry , gene , biology , microbiology and biotechnology , sewage treatment , environmental engineering , biochemistry , environmental science , organic chemistry
In this study, a lab‐scale sequencing batch bioreactor (SBR) with hydraulic retention time (HRT) of 8 h was established to treat 50 mg/L 4‐chlorophenol (4‐CP), and the temporal responses of 4‐CP on the performance and the expression level of related functional genes in sludge were explored. Results showed that after the SBR was acclimated with the cometabolic carbon sources of 4‐CP and methanol, the pollutants were effectively degraded. The pollutants removal was influenced by the polysaccharides and proteins in sludge. The real‐time PCR showed that except for the gene p450 , the prompt removal of pollutants before 2 h caused the significant upregulation of the other functional genes from the genus Pseudomonas in sludge, while the downregulation occurred from 2 to 8 h with the progressive removal of pollutants in SBR cycle, indicating that the removal of pollutants was closely related to the functional genes expression.