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Comparative study of survival of altered and hybrid Vibrio cholerae O1 El Tor in association with Anabaena variabilis in fresh and brackish water microcosms
Author(s) -
Akter Tanzina,
Mahmud Zahid Hayat,
Islam Mohammad Shafiqul,
Mahmud Hafij Al,
Ahmed Mir Alvee,
Rahman Mohammad Hasibur,
Parvez Mohammad Anowar Khasru,
Islam Khairul,
Clemens John David,
Islam Mohammad Sirajul
Publication year - 2017
Publication title -
water and environment journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.437
H-Index - 37
eISSN - 1747-6593
pISSN - 1747-6585
DOI - 10.1111/wej.12282
Subject(s) - brackish water , microcosm , anabaena variabilis , biology , el tor , brackish marsh , vibrio cholerae , microbiology and biotechnology , viable but nonculturable , bacteria , cyanobacteria , salinity , ecology , genetics
The survival of altered and hybrid Vibrio cholerae O1 El Tor in association with a cyanobacterium, Anabaena variabilis in pond (fresh) and brackish water microcosms was investigated. Survival of culturable bacteria was monitored on taurocholate tellurite gelatine agar plate. Viable but nonculturable (VBNC) bacteria were examined using fluorescent antibody (FA) technique. In pond water, altered and hybrid El Tor survived with cyanobacteria 10 and 18 days respectively and in control water 6 and 12 days respectively in culturable state. In pond water VBNC state altered and hybrid El Tor were found to persist 48 and 53 days respectively but in brackish water 65 and 70 days respectively in association with cyanobacteria. Brackish water was found to be a better habitat than pond water for the survival of these strains. Hybrid El Tor survived longer inside the mucilaginous sheath of A. variabilis than altered El Tor both in pond and brackish water microcosms.