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Exploring bioaugmentation strategies for azo dye CI Reactive Violet 5 decolourization using bacterial mixture: dye response surface methodology
Author(s) -
Ayed Lamia,
Bekir Karima,
Achour Sami,
Cheref Abdelkrim,
Bakhrouf Amina
Publication year - 2017
Publication title -
water and environment journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.437
H-Index - 37
eISSN - 1747-6593
pISSN - 1747-6585
DOI - 10.1111/wej.12216
Subject(s) - bioaugmentation , biodegradation , chemistry , microbial consortium , response surface methodology , reactive dye , chemical oxygen demand , degradation (telecommunications) , bacterial adhesin , microbiology and biotechnology , chromatography , food science , environmental chemistry , bacteria , environmental engineering , escherichia coli , wastewater , bioremediation , biology , organic chemistry , biochemistry , environmental science , gene , microorganism , dyeing , computer science , telecommunications , genetics
The use of response surface methodology based on statistical design of experiments is becoming increasingly widespread in several sciences such as analytical and environmental chemistry. In the present study, the decolourization and the degradation efficiency of CI Reactive Violet 5 (CIRV5) was studied using a novel bacterial consortium. CIRV5 (1000 p.p.m.) biodegradation was investigated under shaking condition in mineral salt medium solution (MSM) at a 7.5 pH and a 25°C temperature. The degradation pathways were also predicted, using UV‐visible spectroscopy analysis. Under optimal conditions, the bacterial consortium was able to decolourize the dye completely (>99%) within 8 h. The colour and chemical oxygen demand (COD) removal were 99.29 and 94.93%, respectively. Polymerase chain reaction (PCR) technique was utilized to detect the adhesin genes ‘ ica A’ and ‘ ica D.’ Our results showed that Staphylococcus aureus had a high decolourization capacity. Phytotoxicity study using Triticum turgidum ssp durum showed the no toxicity of the produced products.