Premium
Ion flux kinetics in blue light‐grown field dodder ( C uscuta campestris ) seedlings
Author(s) -
Haidar Mustapha,
Shabala Sergey
Publication year - 2015
Publication title -
weed biology and management
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.351
H-Index - 36
eISSN - 1445-6664
pISSN - 1444-6162
DOI - 10.1111/wbm.12079
Subject(s) - etiolation , flux (metallurgy) , biophysics , biology , kinetics , blue light , ion , vanadate , ion transporter , membrane , seedling , botany , biochemistry , chemistry , materials science , enzyme , physics , optoelectronics , organic chemistry , quantum mechanics
Previous studies have revealed that blue light stimulates coiling and prehaustoria development in young de‐etiolated dodder seedlings prior to host attachment. In this study, the C a 2+ and H + flux kinetics that are associated with blue light‐mediated coiling and prehaustoria development in de‐etiolated dodder seedlings were measured uninvasively using the ion‐selective, vibrating microelectrode ion flux system. The findings showed that blue light induces rapid transient changes in the net C a 2+ and H + fluxes. A clearly pronounced lag of about several minutes was observed between the blue light‐induced changes in the C a 2+ and H + fluxes, with H + being a leading ion. The addition of 1 mol L −1 vanadate, a known blocker of the plasma‐membrane H + ‐ ATP ase, to the bathing solution completely prevented both the H + and the C a 2+ flux responses and inhibited coiling and prehaustoria development, suggesting a causal relationship between the blue light‐induced changes in the net C a 2+ and H + fluxes and seedling movements. It is concluded that the plasma‐membrane H + ‐ ATP ase plays a key role in a dodder's parasitic mode of growth and its adaptive response to the environment.