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Resistin concentrations in serum and stifle synovial fluid from normal and cruciate deficient dogs with osteoarthritis
Author(s) -
Kleine Stephanie A.,
Gogal Robert M.,
Krunkosky Thomas M.,
Sanderson Sherry L.,
George Clinton,
Norton Megan,
Budsberg Steven C.
Publication year - 2020
Publication title -
veterinary surgery
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.652
H-Index - 79
eISSN - 1532-950X
pISSN - 0161-3499
DOI - 10.1111/vsu.13450
Subject(s) - resistin , medicine , peripheral blood mononuclear cell , synovial fluid , adipokine , osteoarthritis , endocrinology , stifle joint , cruciate ligament , leptin , obesity , pathology , surgery , in vitro , biology , anterior cruciate ligament , biochemistry , alternative medicine
Objective To compare synovial fluid (SF) resistin concentrations in healthy dogs to dogs with osteoarthritis (OA) secondary to cranial cruciate ligament (CrCL) injury and to correlate resistin concentrations with body condition score (BCS) and evaluate resistin release from peripheral blood mononuclear cells (PBMC) and adipocytes. Study design Controlled, prospective, clinical study Animals Thirty‐nine client‐owned dogs, 13 healthy and 26 with secondary OA, were enrolled. Blood was collected from six healthy purpose‐bred dogs for PBMC culture. An additional six mixed‐breed dogs were used for adipocyte collection and culture. Methods Resistin concentrations were measured with a canine‐specific enzyme‐linked immunoabsorbent assay. Resistin was compared between healthy SF and OA SF with Student's t test. Correlation of resistin concentrations to BCS was performed. Peripheral blood mononuclear cells and adipocytes were cultured under three conditions: negative control, lipopolysaccharide, and concanavalin A (Con A). A linear mixed model was used to determine differences in resistin concentrations among treatments. Results Resistin concentrations in OA SF were comparable to healthy SF. Neither serum nor SF resistin was correlated with BCS. Cultured PBMC stimulated with Con A released resistin, while adipocytes did not. Conclusion Neither serum nor SF resistin were altered in dogs with OA secondary to CrCL insufficiency. In addition, resistin was not correlated with canine body fat and did not appear to function as adipocytokine in the dog. Clinical significance Resistin may not be involved in the pathogenesis of OA. However, resistin may be important in inflammation because it is released from inflammatory cells.