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Correlation of serum and synovial leptin concentrations with body condition scores in healthy and osteoarthritic dogs
Author(s) -
Kleine Stephanie A.,
Sanderson Sherry L.,
George Clinton,
Roth Ira,
Gogal Robert M.,
Thaliath Mary Ann,
Budsberg Steven C.
Publication year - 2019
Publication title -
veterinary surgery
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.652
H-Index - 79
eISSN - 1532-950X
pISSN - 0161-3499
DOI - 10.1111/vsu.13244
Subject(s) - medicine , leptin , synovial fluid , osteoarthritis , overweight , obesity , cruciate ligament , endocrinology , population , prospective cohort study , gastroenterology , pathology , surgery , anterior cruciate ligament , alternative medicine , environmental health
Objective To evaluate the relationship between serum and synovial fluid (SF) leptin concentrations and body condition score (BCS) in healthy and osteoarthritic dogs. Study design Controlled, prospective, clinical study. Animals Nineteen healthy dogs and 29 dogs with osteoarthritis (OA) secondary to cranial cruciate ligament injury .Methods Synovial fluid was obtained from the femorotibial joint under sedation (healthy dogs) or during surgery (OA dogs). Serum and SF leptin and interleukin (IL)‐1β concentrations were measured via enzyme‐linked immunosorbent assay. Dogs were classified as optimal weight (BCS 4–5/9) or overweight (BCS >5/9). Radiographs were scored for OA severity by a radiologist. Owners completed the Liverpool Osteoarthritis in Dogs (LOAD) questionnaire. Results Mean (± SD) SF leptin (4.09 ± 4 ng/mL) was lower than serum leptin (6.88 ± 5.52 ng/mL, P < .0001). Synovial fluid leptin was higher in overweight (5.28 ± 4.21) than in optimal body weight dogs (1.54 ± 1.72 ng/mL, P < .0001). Serum ( P < .001) and SF leptin ( P = .004) concentrations were associated with BCS. Concentration of SF leptin did not differ between healthy (2.4 ± 2.04 ng/mL) and OA (4.9 ± 4.3 ng/mL, P = .25) dogs. Synovial fluid leptin and LOAD scores were weakly associated ( P = .03). No association was detected between SF leptin and radiographic score or IL‐1β ( P = .73). Conclusion Serum and SF leptin correlated with BCS in this population. Synovial fluid leptin was weakly associated with LOAD scores but not with radiographic severity of OA or IL‐1β. Clinical significance Serum and SF leptin concentrations do not predict radiographic severity of canine OA but contribute to joint pain and dysfunction.