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Influence of intravenous regional limb perfusion with amikacin sulfate on Staphylococcus aureus bioburden in distal limb wounds in horses
Author(s) -
Freeland Russell B.,
Morello Samantha L.,
DeLombaert Melissa,
Rajamanickam Victora
Publication year - 2017
Publication title -
veterinary surgery
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.652
H-Index - 79
eISSN - 1532-950X
pISSN - 0161-3499
DOI - 10.1111/vsu.12638
Subject(s) - medicine , bioburden , limb perfusion , cephalic vein , forelimb , amikacin , perfusion , staphylococcus aureus , vein , surgery , anesthesia , antibiotics , anatomy , microbiology and biotechnology , genetics , biology , bacteria
Objective To quantify the influence of intravenous regional limb perfusion (IVRLP) with amikacin on bacterial bioburden in limb wounds. Study design In vivo, experimental. Animals Four adult horses. Methods Full thickness wounds created on each dorsal metacarpus were inoculated with Staphylococcus aureus . One forelimb was randomly assigned to cephalic vein IVRLP with amikacin on days 2, 3, and 4 post‐wounding. Biopsies on day 2 prior to IVRLP, and on days 5 and 8 were cultured to quantify wound bioburden (CFU/g). Two horses repeated the study in a crossover design, receiving IVRLP on the opposite limb (n = 6 experiments). IVRLP was performed on both forelimbs simultaneously in 3 horses at the end of the study: limbs were perfused with a volume of new methylene blue equal to that of the amikacin, through the cephalic vein or palmar digital vein. After euthanasia, wounds were photographed to subjectively assess dye diffusion. Results The bioburden did not differ between control and IVRLP limbs overall or at any individual time point. No difference was detected between groups in terms of frequency of positive bacterial growth at any time. Methylene blue was visible in all excised tissues after IVRLP through the palmar digital vein, but was not visible in limbs perfused through the cephalic vein. Conclusion IVRLP may not effectively concentrate amikacin within a wound bed and did not influence S. aureus bioburden in an experimentally created wound infection.