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Assessment of tuber coxae bone biopsy in the standing horse
Author(s) -
Mitchell Colin F.,
Richbourg Heather A.,
Goupil Brad A.,
Gillett Ashley N.,
McNulty Margaret A.
Publication year - 2017
Publication title -
veterinary surgery
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.652
H-Index - 79
eISSN - 1532-950X
pISSN - 0161-3499
DOI - 10.1111/vsu.12603
Subject(s) - medicine , biopsy , histology , horse , bone biopsy , radiology , pathology , nuclear medicine , biology , paleontology
Objective To describe a biopsy technique in standing horses with minimal morbidity that consistently provides a substantial bone biopsy with intact, undamaged architecture. Study Design Experimental, prospective study. Animals Ten Thoroughbred horses. Methods Biopsies were obtained from the tuber coxae of 10 sedated, standing horses using an oscillating saw. Bilateral biopsies, separated by 60 days, were evaluated with micro‐computed tomography (microCT). The first biopsy was prepared for decalcified histology; the second for undecalcified histology. Both biopsies were evaluated qualitatively for histologic quality. Results The biopsy technique did not result in any significant complications, was well tolerated and all biopsies were of good histologic quality. Conclusion Cortical and trabecular bone biopsies can be successfully collected from the tuber coxa using a simple technique that creates minimal morbidity and allows sequential samples to be collected. The biopsies were larger than those described previously, provided adequate bone for multiple histologic sections, and had intact, undamaged architecture on examination with microCT and light microscopy.