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Biofilm‐Associated Gene Expression in Staphylococcus pseudintermedius on a Variety of Implant Materials
Author(s) -
Crawford Evan C.,
Singh Ameet,
Gibson Thomas W.G.,
Weese J. Scott
Publication year - 2016
Publication title -
veterinary surgery
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.652
H-Index - 79
eISSN - 1532-950X
pISSN - 0161-3499
DOI - 10.1111/vsu.12471
Subject(s) - staphylococcus pseudintermedius , biofilm , microbiology and biotechnology , gene expression , gene , real time polymerase chain reaction , medicine , biology , bacteria , staphylococcus , staphylococcus aureus , genetics
Objective To evaluate the expression of biofilm‐associated genes in Staphylococcus pseudintermedius on multiple clinically relevant surfaces. Study Design In vitro experimental study. Sample Population Two strains of methicillin‐resistant S. pseudintermedius isolated from clinical infections representing the most common international isolates. Methods A quantitative polymerase chain reaction (qPCR) assay for expression of genes related to biofilm initial adhesion, formation/maturation, antimicrobial resistance, and intracellular communication was developed and validated. S. pseudintermedius biofilms were grown on 8 clinically relevant surfaces (polymethylmethacrylate, stainless steel, titanium, latex, silicone, polydioxanone, polystyrene, and glass) and samples of logarithmic and stationary growth phases were collected. Gene expression in samples was measured by qPCR. Results Significant differences in gene expression were identified between surfaces and between bacterial strains for most gene/strain/surface combinations studied. Expression of genes responsible for production of extracellular matrix were increased in biofilms. Expression of genes responsible for initial adhesion and intracellular communication was markedly variable. Antimicrobial resistance gene expression was increased on multiple surfaces, including stainless steel and titanium. Conclusion A method for evaluation of expression of multiple biofilm‐associated genes in S. pseudintermedius was successfully developed and applied to the study of biofilms on multiple surfaces. Variations in expression of these genes have a bearing on understanding the development and treatment of implant‐associated biofilm infections and will inform future clinical research.