Open AccessMetagenomics in virus discovery
Author(s) -
Jazaeri Farsani S. M.,
Oude Munnink B. B.,
Deijs M.,
Canuti M.,
Hoek L.
Publication year - 2013
Publication title -
isbt science series
Language(s) - English
Resource type - Journals
eISSN - 1751-2824
pISSN - 1751-2816
DOI - 10.1111/voxs.12039
Subject(s) - metagenomics , biology , genome , virus , amplified fragment length polymorphism , virology , computational biology , dna sequencing , whole genome sequencing , population , genetics , gene , medicine , genetic diversity , environmental health
Virus infections are a continuous threat to the human population. Apart from infections with known viruses, there is a world of yet unknown viruses which may cause new emerging diseases, or cause known infectious diseases. Discovery of unknown viruses requires serious efforts since there is no universal viral genomic sequence to amplify an unknown viral genome, as is the case for bacteria for which 16S rRNA sequences can be used. In the last decennium, next generation sequencing allowed new approaches for virus discovery. Our virus discovery method is based on cDNA‐AFLP (AFLP: Amplified Fragment Length Polymorphism) combined with next generation sequencing techniques (VIDISCA‐454). The method supplies thousands of sequence reads with which any RNA or DNA virus in patient samples can be detected.