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Allogeneic major histocompatibility complex antigens are necessary and sufficient for partial tolerance induced by transfusion of pathogen reduced platelets in mice
Author(s) -
Tran Johnson Q.,
Muench Marcus O.,
Heitman John W.,
Jackman Rachael P.
Publication year - 2019
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/vox.12756
Subject(s) - immunology , antigen , major histocompatibility complex , minor histocompatibility antigen , histocompatibility , biology , human leukocyte antigen
Background And Objectives Alloimmunization is common following transfusion with platelet‐rich plasma ( PRP ) and can cause complications such as platelet refractoriness or transplant rejection. It has previously been shown that pathogen reduction of PRP with riboflavin and UV light ( UV +R) can protect against alloimmunization in mice and induce partial tolerance to subsequent transfusions. Materials and Methods Using B6 H2 d congenic mice, this study evaluated the relative contributions of major histocompatibility complex ( MHC ) antigens and minor antigens to both the alloresponse to PRP transfusion and the partial tolerance induced by UV +R treatment. Results Both total and MHC ‐specific alloantibody responses were highest when both MHC and minor antigens were mismatched, with lower alloantibody responses observed with MHC mismatch alone, demonstrating that allogeneic minor antigens can enhance the response to allogeneic MHC . There was a weak, but significant alloantibody response to minor antigens only. UV +R treatment protected against both major and minor antigen alloimmunization. Both allogeneic MHC and minor antigens primed an enhanced cytokine response ex vivo , though this was weaker with minor antigens, and both responses were blocked with UV +R treatment. Conclusion Allogeneic MHC is both necessary and sufficient to induce the partial tolerance associated with UV +R treatment.