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Platelet storage properties are associated with donor age: in vitro quality of platelets from young donors and older donors with and without Type 2 diabetes
Author(s) -
Bontekoe Ido J.,
Meer Pieter F.,
Verhoeven Arthur J.,
Korte Dirk
Publication year - 2019
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/vox.12739
Subject(s) - platelet , buffy coat , type 2 diabetes , whole blood , medicine , in vitro , diabetes mellitus , andrology , chemistry , immunology , endocrinology , biochemistry
Background and Objectives Previously it has been shown that platelet ( PLT ) storage performance is consistent by donor. Differences involved metabolic activity, which might be caused by mitochondrial (dys)function, associated with age and age‐related diseases like Type 2 diabetes (T2D). We aimed to test PLT s from young donors in comparison with PLT s from older donors with or without diagnosis for T2D. Materials and methods Fifteen whole blood donors <30 year were selected, and single‐donor platelet concentrates ( sPC ) were prepared from buffy coats ( BC ) and plasma. Also, 2 × 11 sPC were prepared from matched donors >45 years with and without T2D. The sPC were stored for 8 days and analysed at regular intervals for in vitro quality. Results Donors were 24 ± 3, 60 ± 7 (without T2D) and 59 ± 8 (with T2D) years old. All sPC groups had comparable volume and PLT content. On Day 8, sPC from young donors showed higher pH 37°C than sPC from older donors (6.84 ± 0.15 vs. 6.40 ± 0.48, P < 0.01), due to lower lactate production. Also, CD 62P expression (22.9 ± 7.4 vs. 48.8 ± 24.0%, P < 0.01) and HSR reflected better in vitro quality. PLT storage properties of sPC obtained from T2D donors ( pH = 6.51 ± 0.35) were not different from sPC of matched donors ( pH = 6.40 ± 0.48). No differences in mitochondrial membrane potential were detected between the groups. Conclusion Platelets from young donors exhibited the best storage conditions. On average, PLT s from older donors showed poorer in vitro quality but, considering the sub‐optimal storage conditions, the implications for the daily blood bank routine is probably small. No association with T2D was found.