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Safety of leucodepleted salvaged blood in oncological surgery: an in vitro model
Author(s) -
Marraccini C.,
Merolle L.,
Berni P.,
Boito K.,
Tamagnini I.,
Kuhn E.,
Ragazzi M.,
Baricchi R.,
Pertinhez T. A.
Publication year - 2017
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/vox.12565
Subject(s) - medicine , flow cytometry , immunohistochemistry , blood transfusion , metastasis , colorectal cancer , surgery , blood cell , cancer , pathology , immunology
Intra‐operative blood cell salvage ( IOCS ) is mainly avoided in onco surgery due to the suspicion that it could increase metastasis’ risk. We simulated IOCS followed by leucodepletion: HCT 116 (human colorectal cancer) cells were inoculated into packed red blood cells units, and their distribution was evaluated, step‐by‐step, by flow cytometry and immunohistochemistry. Most of HCT 116 cells were lost during washing, and almost completely removed after filtration. IOCS plus leucodepletion could be of great advantage for oncological patients, where allogenic blood transfusion could influence tumour progression.