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Early γ‐irradiation and subsequent storage of red cells in SAG ‐M additive solution potentiate energy imbalance, microvesiculation and susceptibility to stress‐induced apoptotic cell death
Author(s) -
Qadri S. M.,
Chen D.,
Schubert P.,
Devine D. V.,
Sheffield W. P.
Publication year - 2017
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/vox.12518
Subject(s) - phosphatidylserine , apoptosis , irradiation , programmed cell death , red blood cell , cytosol , andrology , oxidative stress , chemistry , biophysics , biology , immunology , microbiology and biotechnology , biochemistry , membrane , medicine , enzyme , phospholipid , physics , nuclear physics
γ‐Irradiation of red blood cell ( RBC ) concentrates prevents transfusion‐associated graft‐versus‐host disease but may diminish RBC quality. Herein, we show that early γ‐irradiation (25 Gy) of RBC units and their subsequent storage in SAG ‐M additive solution altered membrane microvesiculation, supernatant haemoglobin and cytosolic ATP . γ‐Irradiation did not influence phosphatidylserine externalization, a marker of erythrocyte apoptotic cell death (eryptosis), in RBC stored for 42 days. However, shorter periods (4–21 days) of storage accentuated eryptosis in γ‐irradiated RBC versus untreated RBC s following energy depletion, suggesting that γ‐irradiated RBC is primed for stress‐induced eryptosis during storage.