Premium
Association of HLA ‐ DRB 1 and HLA ‐ DQB 1 with red‐blood‐cell alloimmunization in the Czech population
Author(s) -
Maluskova A.,
Mrazek F.,
Pauliskova M.,
Kovarova P.,
Koristka M.,
Jindra P.,
Cermakova Z.
Publication year - 2017
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/vox.12478
Subject(s) - human leukocyte antigen , immunology , population , microbiology and biotechnology , antigen , biology , medicine , environmental health
Background and Objectives Alloimmune antibodies against red‐blood‐cell ( RBC ) antigens induced in susceptible individuals (responders) by transfusion, pregnancy or transplantation may have serious clinical consequences. The aim of this study was to investigate association of alloimmunization against selected RBC antigens with HLA ‐Class II . Materials and Methods A total of 230 responders (106 monoresponders and 124 multiresponders) were enrolled into the study. HLA ‐ DRB 1 and HLA ‐ DQB 1 variants were determined by PCR ‐ SSO and their frequencies compared between the patients (patient subgroups) and 375 ethnically and regionally matched controls. Results Development of multiple RBC antibodies was associated with HLA ‐ DRB 1*15 and HLA ‐ DQB 1*06 allelic groups in the patients, with the relationship being particularly apparent in those with anti‐C+D antibodies. Furthermore, DRB 1*13 and DQB 1*06 were more frequent in multiresponders with anti‐E+c antibodies and DRB 1*03 and DQB 1*02 in those with anti‐E+Cw. Conclusion For the first time, we confirmed the association of HLA ‐ DRB 1*15 with RBC antibody multiresponder status and found HLA ‐Class II associations for three frequent RBC antibody combinations. Our data support the concept that HLA restriction plays an important role in the response to RBC alloantigens.