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Critical re‐appraisal of blood component quality after overnight hold of whole blood outside current room temperature limits
Author(s) -
Bontekoe I. J.,
Meer P. F.,
Korte D.
Publication year - 2017
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/vox.12474
Subject(s) - buffy coat , blood component , whole blood , haemolysis , blood bank , platelet , medicine , blood preservation , chemistry , zoology , andrology , surgery , immunology , biology , intensive care medicine , medical emergency
Background and Objectives According to European guidelines, the temperature of whole blood ( WB ) has to be maintained at 20–24°C until processing within 24 h, but in blood bank practice, WB is frequently held at temperatures between 18–25°C. We aimed to assess the impact of these small temperature deviations on the quality of the blood components. Materials and Methods After rapid cooling, 7 WB units were held overnight at 18°C and 8 units at 25°C, reflecting worst case holding conditions, and separated into a red cell concentrate ( RCC ), plasma and buffy coat ( BC ). RCC s were filtered at test temperature and stored for 42 days at 2–6°C. BC s were processed to single‐ BC platelet concentrates ( sPC ) and stored up to Day 8 at 20–24°C. Results After overnight hold at 18°C, 2,3‐ DPG in WB decreased by 34 ± 9%, while at 25°C the decrease was 82 ± 6%. Accordingly, the 2,3‐ DPG levels in the RCC s in the 25°C group were significantly lower than in the 18°C group (2·2 ± 1·4 vs. 10·4 ± 2·9 μmol/g Hb). RCC s and sPC s in the 25°C group showed higher initial lactate levels and lower pH compared to the 18°C group, but these differences levelled off at the end of storage. RCC s showed small differences in ATP levels and haemolysis. Plasma in both groups showed comparable Factor VIII :C levels. Conclusion The temperature of WB during overnight hold strongly affects initial 2,3‐ DPG levels of RCC s and supports the maintenance of temperature limits between 20 and 24°C. Other in vitro effects of the temperature deviations were small and of no practical relevance.