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Clinical utility of the basophil activation test for analysis of allergic transfusion reactions: a pilot study
Author(s) -
Okamura I.,
Matsuyama N.,
Yasui K.,
Hirayama F.,
Ikeda T.
Publication year - 2017
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/vox.12471
Subject(s) - basophil activation , basophil , allergen , immunology , immunoglobulin e , medicine , cd63 , platelet , allergy , antibody , chemistry , biochemistry , microrna , microvesicles , gene
Background and Objectives In previous studies, we demonstrated that the basophil‐activating effects of supernatants found in residual‐transfused platelet concentrates ( PC ‐ SN s) on whole blood basophils in cases of allergic transfusion reactions ( ATR s) could be assessed by the basophil activation test ( BAT ) in terms of allergen/IgE dependency. However, in these studies, the basophils were derived from third‐party healthy volunteers. In this study, we performed BAT using patients' own blood basophils to analyse ATR s. Materials and Methods The BAT was performed in two cases of severe ATR s using residual PC ‐ SN s and the patients' own basophils in the presence and absence of dasatinib, an inhibitor of IgE‐mediated basophil activation. Results In both cases, PC ‐ SN s exhibited basophil‐activating activity against the patients' basophils, but not against basophils from third‐party healthy volunteers. In addition, basophil activation was inhibited in the presence of dasatinib, indicating that the basophils were activated in an allergen/IgE‐dependent manner. Of note, the basophils in Case 2, but not in Case 1, were activated by PC ‐ SN s from some unrelated non‐haemolytic transfusion reaction cases. Conclusion This pilot study indicates that the BAT may be useful in clarifying the causal relationship between ATR s and transfused blood as well as in elucidating the mechanisms behind ATR s considering the allergen/IgE‐dependent pathway.