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Comparative biochemical studies of fresh frozen plasma and pooled solvent/detergent‐treated plasma (octaplas LG ® ) with focus on protein S and its impact in different thrombin generation assay set‐ups
Author(s) -
Heger A.,
Janisch S.,
Pock K.,
Römisch J.
Publication year - 2016
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/vox.12420
Subject(s) - thrombin , thrombin generation , chemistry , thromboelastometry , protein s , protein c , blood proteins , bradford protein assay , chromatography , biochemistry , microbiology and biotechnology , fibrinogen , platelet , immunology , medicine , biology
Background and Objectives The solvent/detergent treatment enables effective and robust inactivation of all lipid‐enveloped viruses, but also inactivates partly sensitive plasma proteins such as protein S. The aim of this study was to investigate the thrombin generation capacity of octaplas LG ® , in particular focusing on the function of protein S in thrombin generation assay and the impact of assay settings. Materials and Methods Sixteen octaplas LG ® batches and 32 units of single donor fresh frozen plasma ( FFP ) were investigated. For protein S, both functional activity and free antigen levels were measured. Thrombin generation assay was performed using two fluorogenic tests with different triggers. Finally, rotational thromboelastometry was performed. Results Mean protein S levels were lower in octaplas LG ® , but a wider range of values was found for FFP . Clotting parameters and thrombin generation capacities overlapped between the two plasma groups as demonstrated using both thrombin generation assays and different triggers. Spiking studies with protein S‐depleted plasma, human purified protein S or antibodies against protein S confirmed a correlation between protein S and thrombin generation capacity under specific assay conditions, especially in an assay with low tissue factor concentration. Conclusion Correlation between protein S and thrombin generation capacity was demonstrated in the TGA . Due to higher variability in protein S content in the FFP group, overlapping haemostatic potentials of the two plasma groups were found.