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Evaluation of soluble glycoprotein V as an in vitro quality marker for platelet concentrates: a correlation study between in vitro platelet quality markers and the effect of storage medium
Author(s) -
Kiminkinen L. K. M.,
Krusius T.,
Javela K. M.
Publication year - 2016
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/vox.12402
Subject(s) - platelet , apheresis , platelet activation , in vitro , chemistry , whole blood , buffy coat , plateletpheresis , andrology , biochemistry , immunology , medicine
Background and objectives Extent of shape change ( ESC ), hypotonic shock response ( HSR ), the adhesive glycoprotein CD 62P and lactate have been widely used as in vitro quality makers of platelet concentrates. Our aim was to evaluate soluble glycoprotein V ( sGPV ) as a platelet in vitro activation marker for platelet concentrates. Data were obtained from different validations during a twelve‐year period. Materials and methods Platelet concentrates stored in PAS B, PAS E with 20–35% plasma carry‐over or in plasma were prepared either from buffy coats or collected by apheresis. The samples were analysed up to day seven using sGPV , ESC , HSR , CD 62P and lactate in addition to normal quality control assays. Results sGPV correlated statistically significantly with ESC , HSR , CD 62P and lactate in platelet concentrates stored in PAS s. Conclusion We propose that quantitative sGPV which is calculated per platelet number could be used as the first choice in vitro platelet activation marker in validations and monitoring platelet production processes. sGPV could also be a promising alternative to platelet activation assays used in interlaboratory comparisons of the quality of platelet concentrates. Quantitative lactate and lactate production rate can be used to evaluate the quality of platelets concentrates during storage. However, the formation of lactate depends on the platelet count and the amount of glucose in the product making the comparison more difficult.