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Further studies to evaluate methods of leucoreduction to prevent alloimmune platelet refractoriness and induce tolerance in a dog platelet transfusion model
Author(s) -
Slichter S. J.,
Abrams K.,
Bailey S. Lawrence,
Pellham E.,
Gettinger I.,
Christoffel T.,
Gaur L.,
Latchman Y.,
Nelson K.,
Bolgiano D.
Publication year - 2016
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/vox.12388
Subject(s) - platelet , medicine , platelet transfusion , buffy coat , refractory period , immunology
Objectives Three leucoreduction filters were evaluated – when used alone or combined with centrifuge leucoreduction (C‐ LR ) – to prevent alloimmune platelet refractoriness in a dog platelet transfusion model. Materials and Methods Donor platelet‐rich plasma ( PRP ) or buffy coat ( BC ) platelets were either filter leucoreduced (F‐ LR ) or F‐ LR /C‐ LR , 51 Cr radiolabelled and transfused. Weekly transfusions were given for up to 8 weeks or until platelet refractoriness. Recipients who accepted treated transfusions were then given non‐leucoreduced (non‐ LR ) platelets to determine whether donor‐specific tolerance had been induced. Results Acceptance of F‐ LR PRP transfusions ranged from 29% to 66%. F‐ LR /C‐ LR transfusions prepared from PRP were accepted by 92%, from BC by 63% and from pooled PRP by 75% of recipients (p= NS ); overall acceptance rate of F‐ LR /C‐ LR transfusions was 83%. Tolerance to subsequent non‐ LR transfusions occurred in 45% of the F‐ LR ‐/C‐ LR ‐accepting recipients unrelated to DR ‐B compatibility between donors and recipients ( P = 0·18). Conclusion In a dog platelet transfusion model, acceptance of donor platelets required combining F‐ LR with C‐ LR as apparently each process removes different immunizing WBC s.