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Influence of early irradiation on in vitro red blood cell ( RBC ) storage variables of leucoreduced RBC s in additive solution SAG ‐M
Author(s) -
HauckDlimi B.,
Braun T.,
Eckstein R.,
Strobel J.,
Zimmermann R.
Publication year - 2016
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/vox.12369
Subject(s) - irradiation , haemolysis , blood irradiation therapy , andrology , mannitol , lactate dehydrogenase , chemistry , biology , biochemistry , immunology , medicine , enzyme , physics , nuclear physics
Background The only accepted way to avoid transfusion‐associated graft‐versus‐host disease is irradiation of blood components. With respect to irradiation and associated storage time, different recommendations exist. We examined early irradiated (day +3) leucoreduced red blood cell units for irradiation‐associated damages during storage. Study Design and Methods We studied 80 leucoreduced units from two manufacturers. All RBC s were stored in the additive solution saline‐adenine‐glucose‐mannitol (SAG‐M) and leucoreduced on collection day. Forty components were irradiated on day +3 with 30 Gy, 40 served as non‐irradiated controls. Samples were drawn and analysed from these 80 units on days +3, +7, +14, +21, +28, +35 and +42. Results From day +14, there was a significant difference in the in vitro haemolysis rate between the non‐irradiated and the irradiated components. Two of the irradiated units showed a haemolysis rate over the recommended limit of 0·8% on day +42. Potassium and lactate dehydrogenase activity increased faster in irradiated groups during storage. Conclusions Our findings show that leucoreduced RBC s which are irradiated early after collection show an obvious radiation damage over storage period. Interestingly, on days +28, +35 and +42 the quality of RBC s which were irradiated on day +3 was nearly identical to the quality of RBC s which were irradiated on day +14 in a former series. Early irradiation does not cause more damage of RBC s during subsequent storage than irradiation on day +14. The maximum storage period of irradiated RBC s should remain restricted to 28 days from collection, however independently from the day of irradiation within this period.