Premium
Additive solutions differentially affect metabolic and functional parameters of platelet concentrates
Author(s) -
Leitner G. C.,
List J.,
Horvath M.,
Eichelberger B.,
Panzer S.,
JilmaStohlawetz P.
Publication year - 2016
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/vox.12317
Subject(s) - platelet , lactate dehydrogenase , platelet activation , chemistry , blood product , p selectin , thrombin , mean platelet volume , medicine , endocrinology , biochemistry , andrology , enzyme , surgery
Background Pathogen inactivation ( PI ) of platelet concentrates with extension of shelf life to 7 days requires the use of platelet additive solutions ( PAS ). We examined the quality of platelets resuspended in three different PAS stored for up to 7 days. Materials and Methods Twelve triple adult dose platelet concentrates ( PC ) were collected using the TrimaAccel ® collection system. Each highly concentrated product was divided into three equal parts, and the additive solutions (Composol ® or SSP + ® or Intersol ™ ) were added to a final concentration of 56% PAS and 44% plasma. Samples were drawn on days 1, 5 and 7 to measure pH , glucose, lactate dehydrogenase ( LDH ), lactate, mean platelet volume ( MPV ) and the aggregation response to collagen and the thrombin receptor agonist peptide‐6. Further, p‐selectin expression on platelets was assessed. Results No statistically significant changes were observed for pH and MPV during 7 days of storage in all PAS containing PC s, whereas glucose decreased and LDH and lactate increased over time ( P < 0·05). These changes were particularly evident in Intersol PC s on days 5 and 7 compared with Composol ® PC s or SSP + ® PC s ( P < 0·05). Platelets from Intersol PC s exhibited the highest baseline activation of p‐selectin and showed reduced collagen‐ and TRAP ‐6‐induced aggregation. Conclusion Resuspension of platelets in Intersol for 7 days results in increased platelet activation and platelet metabolism compared with SSP + ® or Composol ® . Further clinical studies are needed to evaluate whether the observed differences in PAS ‐ PC s affect the recovery rate or the life span of transfused platelets.