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Stability of coagulation protein activities in single units or pools of cryoprecipitate during storage at 20–24°C for up to 24 h
Author(s) -
Sheffield W. P.,
Bhakta V.,
Jenkins C.
Publication year - 2016
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/vox.12309
Subject(s) - cryoprecipitate , fibrinogen , chemistry , chromatography , dilution , blood preservation , andrology , medicine , biochemistry , physics , thermodynamics
Background and Objective Cryoprecipitate is a concentrated source of fibrinogen and other plasma proteins. Cryoprecipitate must be transfused within 4–6 h of thawing and storage at 20–24°C. We compared plasma protein activities in single or pooled cryoprecipitate units stored at 20–24°C for 0, 4 or 24 h. Materials and Methods Individual cryoprecipitate units ( n = 36) were thawed, diluted with sterile saline and sampled over time. Cryoprecipitate pools of eight individual units were assembled either by serial passage of diluent (Method A, n = 6 pools) or by separate dilution into a single collection bag (Method B, n = 6 pools). Fibrinogen, factor VIII , factor XIII and von W illebrand factor activities were measured. Results No significant losses in activities were found relative to at‐thaw values after either 4 or 24 h of storage of individual cryoprecipitate units at 20–24°C; 35 of 36 units contained >150 mg of fibrinogen. No significant differences were found between activities in single vs. pooled units of cryoprecipitate assembled using either method, or between cryoprecipitate pools made by Method A (80–160 ml volume) or Method B (160–240 ml volume) at 0, 4 or 24 h post‐thaw; freezing and thawing of pools did not lead to significant activity losses. Conclusion The stability of fibrinogen and other factors in thawed cryoprecipitate stored at 20–24°C suggests that the shelf life may be safely extended to 24 h provided that sterility is maintained.