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Automated isolation of primary antigen‐specific T cells from donor lymphocyte concentrates: results of a feasibility exercise
Author(s) -
Bunos M.,
Hümmer C.,
Wingenfeld E.,
Sorg N.,
Pfirrmann V.,
Bader P.,
Seifried E.,
Bönig H.
Publication year - 2015
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/vox.12291
Subject(s) - population , antigen , adoptive cell transfer , immunology , medicine , t cell , immune system , environmental health
Background The safety and clinical efficacy of adoptive transfer of prospectively isolated antigen‐specific T cells are well established. Several competing selection methods are available, one of which is based on immunomagnetic enrichment of T cells secreting IFN γ after incubation with the relevant antigen. The proprietary, GMP ‐conforming selection technology, called ‘cytokine capture system’ ( CCS ) is established in many laboratories for the Clini MACS Plus system. It is robust and efficient, but labour‐intensive and incompatible with a single‐shift working schedule. An automatic immunomagnetic cell processing system, Clini MACS Prodigy (‘Prodigy’), including a protocol for fully automatic CCS execution was recently released. Material and methods Feasibility of clinical‐scale CMV ‐specific T‐cell selection using Prodigy was evaluated using leukoapheresis products from five healthy CMV sero‐positive volunteers. Clinical reagents and consumables were used throughout. Results The process required no operator input beyond set‐up and QC ‐sample collection, that is, feasibility was given. An IFN γ‐secreting target T‐cell population was detectable after stimulation, and >2 log‐scale relative depletion of not CMV ‐reactive T cells in the target population was achieved. Purity, that is the frequency of CMV ‐reactive T cells among all CD 3 + cells ranged between 64 and 93%. Conclusion The CCS protocol on Prodigy is unrestrictedly functional. It runs fully automatically beyond set‐up and thus markedly reduces labour. The quality of the products generated is similar to products generated with Clini MACS Plus. The automatic system is thus suitable for routine clinical application.