Premium
T‐cell subsets in autologous and allogeneic peripheral blood stem cell concentrates
Author(s) -
Strobel J.,
Moellmer I.,
Zingsem J.,
HauckDlimi B.,
Eckstein R.,
Strasser E.
Publication year - 2015
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/vox.12289
Subject(s) - apheresis , stem cell , immunology , medicine , peripheral blood , transplantation , cell , t cell , andrology , biology , immune system , platelet , microbiology and biotechnology , genetics
Background and Objectives Regulatory T cells ( T regs) and other T ‐cell subsets are of importance in the setting of autologous and allogeneic stem cell transplantations. We conducted a study to assess the content of peripheral blood stem cell concentrates and related apheresis parameters in the autologous and allogeneic setting. Material and Methods We characterized 53 donors, patients and peripheral blood stem cell concentrates ( PBSC ) regarding the content of CD 45 + cells, lymphocytes, CD 3 + cells, CD 3 + CD 4 + T cells, CD 3 + CD 4 + CD 25 + T cells, CD 3 + CD 4 + CD 25 + CD 127 low/negative Tregs and CD 34 + cells and calculated cell yields, recruitment factors and collection efficiency for all cell types. We compared allogeneic data with autologous data. Results Autologous PBSC show significantly lower concentrations of T ‐cell subsets compared to allogeneic PBSC (17 112/μl CD 4 + , 14 858/μl CD 4 + CD 25 + and 1579/μl CD 3 + CD 4 + CD 25 + CD 127 low/negative Tregs in autologous compared to 65 539/μl CD 4 + , 44 208 + /μl CD 4 + CD 25 + and 5040/μl CD 3 + CD 4 + CD 25 + CD 127 low/negative Tregs in allogeneic PBSC , respectively), in contrast to CD 34 + concentrations (5342/μl CD 34 + in autologous compared to 2367/μl CD 34 + in allogeneic PBSC , respectively). Accordantly, all T‐cell yields are lower in the autologous setting compared to allogeneic PBSC . However, recruitment factor and collection efficiency of all cell types are higher in autologous compared to allogeneic PBSC , but not all parameters differ significantly when groups are compared. Conclusion T‐cell subsets and especially Tregs are a substantial part of PBSC transplantation, as considerable recruitment during apheresis occurs. In large volume apheresis, the collection efficiency of Treg is comparable to that of CD 34 + cells, while recruitment factors are even higher.