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In vitro evaluation of cytomegalovirus‐specific hyperimmune globulins vs. standard intravenous immunoglobulins
Author(s) -
Miescher S. M.,
Huber T. M.,
Kühne M.,
Lieby P.,
Snydman D. R.,
Vensak J. L.,
Berger M.
Publication year - 2015
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/vox.12246
Subject(s) - cytomegalovirus , intravenous use , antibody , medicine , globulin , intravenous immunoglobulins , immunology , gamma globulin , virology , virus , herpesviridae , viral disease , anesthesia
Background and Objectives To evaluate standard intravenous immunoglobulin ( IVIG ) as an alternative to intravenous cytomegalovirus hyperimmune immunoglobulin ( CMVIG ) for prophylaxis and therapy of cytomegalovirus ( CMV ) disease, we measured the ELISA and neutralizing titres of CMV ‐specific antibodies in CMVIG and IVIG preparations. Materials and Methods Anti‐ CMV ‐IgG ELISA and neutralizing titres (fibroblast‐based test) in CMVIG CG (Cytogam ® , n = 20), CMVIG CT (Cytotect ® CP , n = 3), IVIG P (Privigen ® , n = 32) and IVIG K/G (Kiovig ® /Gammagard ® , n = 5) were compared, and IgG subclasses 1–4 were determined by nephelometry. Results Cytomegalovirus hyperimmune immunoglobulins contained more than fourfold higher CMV ELISA and CMV ‐neutralizing activity per gram of IgG than the standard IVIG s. Pooled data for all four products showed a significant correlation between anti‐ CMV ‐IgG ELISA and neutralizing titres ( r = 0·93, P < 0·001). There was a good correlation between the IgG3 content and CMV ‐neutralizing antibodies amongst lots of CMVIG s ( r = 0·91, P = 0·01), but this did not extend to the IVIG s. CMVIG CG contained the highest CMV ‐neutralizing activity (3497 ± 395 PEIU /g IgG) of any product tested. Conclusion The higher anti‐ CMV neutralization capacity of CMVIG per gram of IgG vs. standard IVIG suggests that standard IVIG s are not equivalent to or interchangeable with CMVIG .