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The effects of an overnight holding of whole blood at room temperature on haemoglobin modification and in vitro markers of red blood cell aging
Author(s) -
Eckstein M.,
Zimmermann R.,
Roth T.,
HauckDlimi B.,
Strasser E. F.,
Xiang W.
Publication year - 2015
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/vox.12235
Subject(s) - red blood cell , malondialdehyde , superoxide dismutase , haemolysis , andrology , catalase , chemistry , oxidative stress , whole blood , red cell , hemolysis , hemoglobin , biochemistry , endocrinology , medicine , immunology , biology
Background Some effects of the red blood cell ( RBC ) storage lesion are well documented whereas others are not. Whether a period of room temperature hold ( RTH ) during RBC production enhances the RBC storage lesion has remained controversial. In this study, we compared whole blood ( WB )‐derived RBC s produced after 24‐h RTH with rapidly cooled ( RC ) RBC s and tested them for classical metabolic markers and signs of oxidative damage. Study design and methods SAGM ‐ RBC s were prepared from mixed and split pairs ( n = 12) of WB units. RBC s prepared after a 24‐h period of RTH on day+1 after collection ( RTH ‐ RBC s) were compared with RC ‐ RBC s. All RBC s were stored at 4°C for 42 days with assay of in vitro variables on days+1, +15, +22, +29 and +42. The study examined standard quality parameters, glutathione, catalase and superoxide dismutase ( SOD ) activities, and indicative markers of oxidative cell damage including post‐translational haemoglobin modification, malondialdehyde (MDA), and phosphatidylserine expression. Results RTH ‐ RBC s exhibited decreased levels of potassium (1·98 ± 0·26 vs. 5·23 ± 0·65 mmol/l) and of 2,3‐diphosphoglycerate (2,3‐ DPG ) on day+1 compared with RC ‐ RBC s. Haemolysis rate on day+42 was higher in RTH ‐ RBC s than in RC ‐ RBC s (0·52 ± 0·13 vs. 0·37 ± 0·12%). The phosphatidylserine expression amounted to 0·25 ± 0·20% in RTH ‐ RBC s and 0·07 ± 0·12% in RC ‐ RBC s. Haemoglobin modification was not different between both RBC groups. RTH ‐ RBC s showed slightly higher MDA concentration on days +29 and +42. Conclusions RC ‐ RBC s and RTH ‐ RBC s show only small differences of classical in vitro parameters and no relevant differences in antioxidative metabolism and oxidative haemoglobin modification. These findings do not explain the loss observed in in vivo survival studies with RBC s.