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Detection of granulocyte‐reactive antibodies: a comparison of different methods
Author(s) -
Heinzl M. W.,
Schönbacher M.,
Dauber E.M.,
Panzer S.,
Mayr W. R.,
Körmöczi G. F.
Publication year - 2015
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/vox.12227
Subject(s) - granulocyte , antibody , immunofluorescence , flow cytometry , antigen , monoclonal antibody , immunology , serology , medicine
Background and Objectives Granulocyte‐reactive antibodies can cause autoimmune and neonatal immune neutropenias as well as transfusion‐related acute lung injury. The classical antibody‐detection methods granulocyte aggregation test ( GAT ), granulocyte immunofluorescence test ( GIFT ) and monoclonal antibody‐specific immobilization of granulocyte antigens ( MAIGA ) are time‐consuming and technically challenging. In recent years, flow cytometric white blood cell immunofluorescence test (Flow‐ WIFT ) and the microbeads assay LabScreen® Multi have emerged and are still subject of evaluation. These serological tests were compared on a screening and specification level. Materials and Methods For screening, the combination of GAT / GIFT was compared to Flow‐ WIFT testing 333 samples. Positive samples were further analysed with MAIGA and LabScreen® Multi. Results Granulocyte aggregation test/ GIFT detected 77 positive samples, Flow‐ WIFT found 108 granulocyte‐reactive samples. Six Samples were only positive in GAT / GIFT , and 37 samples were only positive in Flow‐ WIFT (κ = 0·682). Antibody specification with MAIGA and the microbeads assay confirmed granulocyte‐reactivity in 83 cases with 70 matching results (κ = 0·742). However, out of six detected human neutrophil antigen ( HNA ) reactivities only two specificities matched in both assays. Conclusion Flow‐ WIFT may be a valuable addition to GIFT for granulocyte‐reactive antibody screening. MAIGA remains the most reliable laboratory method for antibody specification.

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