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Quality of functional haematopoietic stem/progenitor cells from cryopreserved human umbilical cord blood
Author(s) -
Eom J.E.,
Kim D. S.,
Lee M. W.,
Yu D.K.,
Jin K. S.,
Shin S.,
Lee S. H.,
Sung K. W.,
Koo H. H.,
Yoo K. H.
Publication year - 2014
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/vox.12132
Subject(s) - cryopreservation , umbilical cord , haematopoiesis , andrology , cord blood , stem cell , immunology , transplantation , bone marrow , cd34 , progenitor cell , biology , medicine , embryo , genetics , microbiology and biotechnology
Background and Objectives Transplantation of cryopreserved umbilical cord blood ( UCB ) can be used to treat a multitude of haematologic and immunological diseases. In this study, we examined the quality of UCB cryopreserved for 2 (group I), 4 (group II ) and 6 (group III ) years. Methods The following parameters and procedures were used to test individual units of cryopreserved UCB : the number of total nucleated cells ( TNC ), cell viability, CFU ‐ GM assay, T‐cell activation in vitro and haematopoietic stem cell engraftment in NOD / SCID mice in vivo . Results The TNC recovery rates for groups I, II and III were 106·2 ± 6·17%, 96·69 ± 6·39% and 100·38 ± 5·27%, respectively, and the mean percentages of viable cells after thawing were 86·88%, 86·38% and 87·43%. When TNC were plated at 5 × 10 3 , the number of CFU ‐GM was 13·6 (group I), 13·8 (group II), 14·2 (group III) and 14·7 (fresh UCB ). We confirmed that the hu CD 4 + and hu CD 8 + T cells within cryopreserved UCB are functionally responsive by assessment of activated hu CD 25 + cells. Moreover, the percentage of hu CD 45 + cells in the bone marrow was 4·32 ± 1·29% (group I), 4·48 ± 1·11% (group II ), 4·40% ± 1·12% (group III ) and 4·50% ± 0·66% (fresh UCB ), and that in the peripheral blood was 14·69 ± 3·08% (group I), 15·24 ± 4·05% (group II ), 15·74 ± 3·43% (group III ) and 17·48 ± 3·74% (fresh UCB ) in NOD / SCID mice infused with isolated hu CD 34 + cells. Conclusion These results indicated that cryopreserved UCB units efficiently retrieve in functionally competent form and are suitable for transplantation.