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A new transfectant panel cell line‐based M o A b‐independent antigen capture assay system for detection of CD 36 antibody
Author(s) -
Amakishi E.,
Hayashi T.,
Koh Y.,
Matsuyama N.,
Ishii H.,
Matsukura H.,
Yasui K.,
Hirayama F.
Publication year - 2014
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/vox.12118
Subject(s) - antigen , monoclonal antibody , antibody , immunology , gold standard (test) , medicine , platelet , microbiology and biotechnology , human leukocyte antigen , biology
Background and Objectives CD 36 antibody ( A b) causes several disorders: neonatal alloimmune thrombocytopenia, platelet transfusion refractoriness and non‐haemolytic transfusion reactions. However, there is no gold‐standard test for CD 36 A b. Materials and Methods We developed a transfectant panel cell line‐based M o A b‐independent antigen capture assay system for detection of CD 36 Ab and compared it with the monoclonal antibody‐specific immobilization of platelet antigens ( MAIPA ) system in terms of sensitivity and specificity. Results Our new system was characterized by (1) gene‐transfected cell lines, but not panel platelets; (2) not being hampered by HLA Abs; and (3) no need to use CD 36 M o A bs to ensure the antigen specificity of this detection system. In addition, it showed a much better receiver operating characteristic curve than the MAIPA system. Conclusions The present results indicate that our new system permits highly sensitive and specific detection of CD 36 A b.