A new transfectant panel cell line‐based M o A b‐independent antigen capture assay system for detection of CD 36 antibody

Background and Objectives CD 36 antibody ( A b) causes several disorders: neonatal alloimmune thrombocytopenia, platelet transfusion refractoriness and non‐haemolytic transfusion reactions. However, there is no gold‐standard test for CD 36 A b. Materials and Methods We developed a transfectant panel cell line‐based M o A b‐independent antigen capture assay system for detection of CD 36 Ab and compared it with the monoclonal antibody‐specific immobilization of platelet antigens ( MAIPA ) system in terms of sensitivity and specificity. Results Our new system was characterized by (1) gene‐transfected cell lines, but not panel platelets; (2) not being hampered by HLA Abs; and (3) no need to use CD 36 M o A bs to ensure the antigen specificity of this detection system. In addition, it showed a much better receiver operating characteristic curve than the MAIPA system. Conclusions The present results indicate that our new system permits highly sensitive and specific detection of CD 36 A b.