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Histologic effects of MicroPulse™ transscleral cyclophotocoagulation in normal equine eyes
Author(s) -
Foote Braidee C.,
Smith Jodi D.,
Allbaugh Rachel A.,
Sebbag Lionel
Publication year - 2021
Publication title -
veterinary ophthalmology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.594
H-Index - 50
eISSN - 1463-5224
pISSN - 1463-5216
DOI - 10.1111/vop.12846
Subject(s) - medicine , ophthalmology , ciliary body , surgery
Objective Determine the immediate post‐operative effects of MicroPulse ™ transscleral cyclophotocoagulation (MP‐TSCPC) in healthy equine eyes. Animals studied Ten adult horses. Methods MP‐TSCPC was performed on sedated horses in 12 eyes (4 groups) using the following parameters (power, duration, duty cycle): (1) 2000 mW, 180 seconds, 31.3%; (2) 3000 mW, 180 seconds, 31.3%; (3) 3000 mW, 270 seconds, 31.3%; and (4) 3000 mW, 270 seconds, 50%. Three additional eyes were left untreated (control). Eyes were monitored clinically until euthanasia (mean = 3 hours post‐procedure). Histologic sections were assessed with light microscopy and transmission electron microscopy (TEM). Results MP‐TSCPC was well tolerated by sedated horses. Adverse effects were only noted in Group 4: ocular hypertension (n = 3/3), conjunctival burns (3/3), aqueous flare (2/3), and a corneal erosion (1/3). Histologic scoring of Group 4 was statistically greater than other treated groups (1‐3) and control eyes ( P ≤ .021). TEM showed subtle changes to the mitochondria and plasma membrane infoldings of the basilar surface of the nonpigmented epithelium in all treated eyes. Conclusions MP‐TSCPC does not cause immediate post‐procedure adverse clinical effects or pronounced morphological changes to the ciliary body, except with the highest laser settings evaluated (power 3000 mW, duration 270 seconds, duty cycle 50%).