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Evaluation of D‐dimer levels in aqueous humor of rabbit eyes with and without induced intraocular fibrin and fibrinolytic treatment
Author(s) -
Voelter Katrin,
Tappeiner Christoph,
Riond Barbara,
Nuss Katja,
Bruetsch Deborah,
Pot Simon A.
Publication year - 2020
Publication title -
veterinary ophthalmology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.594
H-Index - 50
eISSN - 1463-5224
pISSN - 1463-5216
DOI - 10.1111/vop.12706
Subject(s) - fibrin , d dimer , aqueous humor , medicine , dimer , ophthalmology , fibrinolysis , chemistry , surgery , immunology , organic chemistry
Objective To analyze D‐dimer concentrations in aqueous humor (AH) of rabbit eyes under physiological conditions, after induction of fibrin clots, and following fibrinolytic therapy. Animals studied Prospective study measuring D‐dimers in aqueous humor of rabbit eyes with induced fibrin clots (n = 44). Procedures Rabbits were purchased in two groups, which led to two temporally separated experimentation groups. Different treatment protocols were compared for their efficacy in fibrin reduction (slit‐lamp examination, high‐resolution ultrasound). AH was taken from left eyes before clot induction (baseline, day 1), 24 hours later after clot establishment/prior to drug administration (post‐induction, day 2) and 48 hours after clot induction (post‐treatment, day 3). An enzyme‐linked immunosorbent assay (ELISA) was performed to measure intraocular D‐dimer concentrations Results D‐dimer concentrations were measurable in all samples. There were no differences in D‐dimer levels across time points or treatments within the arrival groups. However, a significant difference in mean D‐dimer levels was observed between the two arrival groups (group 1:3.1 µg/mL; group 2:6.1 µg/mL; P < .0001), which made a direct comparison of treatment groups impossible. Clinically, all eyes displayed fibrin clots in the anterior chamber and different treatment types led to significant differences in clot resolution (clot size reduction after intracameral treatment: 98%, topical treatment: 60%, no treatment: 40%). Conclusion D‐dimers were identified in all AH samples of rabbits with large variability between samples. D‐dimer levels were neither predictive for differences in induced fibrin formation nor for drug efficacy.