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Altered fundus appearance resulting from autofluorescence imaging with the confocal scanning laser ophthalmoscope (c SLO ) in cats
Author(s) -
Occelli Laurence M.,
PetersenJones Simon M.
Publication year - 2014
Publication title -
veterinary ophthalmology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.594
H-Index - 50
eISSN - 1463-5224
pISSN - 1463-5216
DOI - 10.1111/vop.12192
Subject(s) - autofluorescence , fundus (uterus) , retinal , confocal , retina , cats , ophthalmology , optical coherence tomography , medicine , fluorescence , optics , physics
Purpose This paper is to report that imaging the tapetal fundus of cats with the 488 nm laser of the S pectralis ® HRA + OCT ( H eidelberg E ngineering I nc., H eidelberg, G ermany) can result in a pale appearance of the imaged area. Animals studied and procedures Wild‐type and R dy kittens ( CRX mutant heterozygotes— CRX Rdy+/− ) (8–20 weeks of age) and adult cats (1–4 years of age) were imaged by confocal scanning laser ophthalmoscope (c SLO ) and spectral domain optical coherence tomography ( SD ‐ OCT ) using the S pectralis ® HRA + OCT . Color fundus photography ( R et C am II ® , C larity M edical S ystems, I nc., P leasanton, CA ) was performed after imaging using the S pectralis ® HRA + OCT . Results Following retinal c SLO imaging using the 488 nm laser (autofluorescence imaging) in both wild‐type kittens and adult cats, the imaged region appeared paler than the adjacent retina that had not been imaged. This change was probably due to retinal bleaching and was fully reversible. Imaging CRX Rdy+/− kittens or adults, which had very reduced levels of visual pigments, did not induce the altered fundus appearance. Conclusions Those using autofluorescence imaging by c SLO should be aware that it can induce a characteristic pale appearance of the tapetal fundus in the imaged area of normal cats.