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Spectral domain optical coherence tomography imaging of the posterior segment of the eye in the retinal dysplasia and degeneration chicken, an animal model of inherited retinal degeneration
Author(s) -
McKibbin Martin,
Ali Manir,
Inglehearn Chris,
Shires Mike,
Boyle Kenny,
Hocking Paul M.
Publication year - 2014
Publication title -
veterinary ophthalmology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.594
H-Index - 50
eISSN - 1463-5224
pISSN - 1463-5216
DOI - 10.1111/vop.12051
Subject(s) - retinal degeneration , optical coherence tomography , degeneration (medical) , retinal , macular degeneration , dysplasia , anatomy , retina , ophthalmology , medicine , biology , pathology , neuroscience
Objective To investigate qualitative and quantitative differences in the structure of the posterior segment of the eye in 1‐day post‐hatch and 12‐month‐old retinal dysplasia and degeneration (rdd) and wild‐type chickens. Animal studied Retinal dysplasia and degeneration and wild‐type chickens. Procedure Using a commercially available spectral domain optical coherence tomography ( OCT ) system, 15° horizontal line scans were performed in both eyes of 24 live birds. Qualitative differences in retinal lamination and choroidal structure were investigated, and retinal and choroidal thickness were measured. Results Progressive retinal thinning with loss of outer retinal lamination and changes in the appearance of the choroid were seen in the rdd birds. Mean total retinal thickness was 202 μm ( SD 7.8) and 251 μm ( SD 8.8) in the rdd and wild‐type chicks and 154 μm ( SD 18) and 280 μm ( SD 10.8) in the adult birds. Much of the difference was the result of loss of outer retinal lamination and thickness in the rdd birds. Mean choroidal thickness was 76 μm ( SD 19.6) and 112 μm ( SD 36.9) in the rdd and wild‐type chicks and 85 μm ( SD 23.7) and 228 μm ( SD 44.1) in the rdd and wild‐type adult birds, respectively. Conclusions Differences in retinal and choroidal structure and thickness between rdd and wild‐type birds were evident on spectral domain OCT imaging at 1‐day post‐hatch and more marked at 1 year. Spectral domain OCT may provide a reliable end point for therapeutic intervention in this animal model of inherited retinal degeneration.